Pectate Lyase Enzyme

A Pectate Lyase Enzyme is an enzyme that is Secreted By Pathogens and initiate Soft-Rot Diseases in Plants by Cleaving Polygalacturonate.

• AKA: Pectate Lyase, Polygalacturonic Transeliminase, Pectic Acid Transeliminase, Polygalacturonate Lyase, Endopectin Methyltranseliminase, Pectate Transeliminase.
• Example(s):
  • Erwinia Carotovora http://www.uniprot.org/uniprot/P0C1C1
  • Erwinia Chrysanthemi http://www.uniprot.org/uniprot/P11073
  • PMID 9515910: “The plant pathogens Erwinia chrysanthemi and Erwinia carotovora secrete several isozymes of pectate lyase (Pel) by the out-encoded type II pathway. ”
• Counter-Example(s):
  • PMID 10361306:“The nucleotide sequence of a 1.6 kb-pair HindIII restriction fragment, which confers pectate lyase activity to E. coli, indicated a 1,260 bp open reading frame encoding a 420 amino acid polypeptide which includes a 21 amino acid signal sequence . The 45,605 Da deduced protein displays homologies to PLs from Erwinia chrysanthemi . The B. subtilis PL cloned in E. coli was located in the periplasm .It was purified to homogeneity in a one-step procedure from the E. coli periplasmic fluid after overproduction using the pT7 system .Biochemical properties of the purified enzyme were similar to those found for the PL isolated from B. subtilis extracellular media”
• See: Erwinia Carotovora.

---

References

2009

• (Wikipedia, 2009) ⇒ http://en.wikipedia.org/wiki/Pectate_lyase
  • In enzymology, a pectate lyase (EC 4.2.2.2) is an enzyme that catalyzes the chemical reaction
  • Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
  • This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is (1->4)-alpha-D-galacturonan lyase. Other names in common use include polygalacturonic transeliminase, pectic acid transeliminase, polygalacturonate lyase, endopectin methyltranseliminase, pectate transeliminase, endogalacturonate transeliminase, pectic acid lyase, pectic lyase, alpha-1,4-D-endopolygalacturonic acid lyase, PGA lyase, PPase-N, endo-alpha-1,4-polygalacturonic acid lyase, polygalacturonic acid lyase, pectin trans-eliminase, and Polygalacturonic acid trans-eliminase. This enzyme participates in pentose and glucuronate interconversions.
• Gene Ontology http://amigo.geneontology.org/cgi-bin/amigo/term-details.cgi?term=GO:0030570&session_id=5828amigo1240506945
  • Accession: GO:0030570
  • Ontology: molecular function
  • Synonyms
    • exact: (1->4)-alpha-D-galacturonan lyase activity
    • exact: alpha-1,4-D-endopolygalacturonic acid lyase activity
    • exact: endo-alpha-1,4-polygalacturonic acid lyase activity
    • exact: endogalacturonate transeliminase activity
    • exact: endopectin methyltranseliminase activity
    • exact: pectate transeliminase activity
    • exact: pectic acid lyase activity
    • exact: pectic acid transeliminase activity
    • exact: PGA lyase activity
    • exact: polygalacturonate lyase activity
    • exact: polygalacturonic acid lyase activity
    • exact: polygalacturonic acid trans-eliminase activity
    • exact: polygalacturonic transeliminase activity
    • exact: PPase-N activity
    • broad: pectic lyase activity
    • broad: pectin trans-eliminase activity
  • Definition
    • Catalysis of the eliminative cleavage of pectate to give oligosaccharides with 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends. [source: EC:4.2.2.2]----