Pullulanase

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(Redirected from EC 3.2.1.41)
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A Pullulanase is a Glucanase, an Amylolytic Exoenzyme, that degrades Pullulan.


References

2019a

  • (Wikipedia, 2019) ⇒ https://en.wikipedia.org/wiki/Pullulanase Retrieved:2019-3-28.
    • Pullulanase (EC 3.2.1.41, limit dextrinase, amylopectin 6-glucanohydrolase, bacterial debranching enzyme, debranching enzyme, alpha-dextrin endo-1,6-alpha-glucosidase, R-enzyme, pullulan alpha-1,6-glucanohydrolase) is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. It is produced as an extracellular, cell surface-anchored lipoprotein by Gram-negative bacteria of the genus Klebsiella. Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. It is also produced by some other bacteria and archaea. Pullulanase is used as a processing aid in grain processing biotechnology (production of ethanol and sweeteners).

      Pullulanase is also known as pullulan-6-glucanohydrolase (Debranching enzyme). Its substrate, pullulan, is regarded as a chain of maltotriose units linked by alpha-1,6-glycosidic bonds. Pullulanase will hydrolytically cleave pullulan (alpha-glucan polysaccharides).

201b

  • (EC, 2019) ⇒ Retrieved:2019-3-28.
    • QUOTE: Reaction catalysed

      Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen

      Comment(s)

2009

  • Gene Ontologyhttp://amigo.geneontology.org/cgi-bin/amigo/term-details.cgi?term=GO:0004629&session_id=5828amigo1240506945
    • Accession: GO:0051060
    • Ontology: molecular function
    • Synonyms
      • related: bacterial debranching enzyme
      • related: R-enzyme
      • exact: alpha-dextrin endo-1,6-alpha-glucosidase activity
      • exact: amylopectin 6-glucanohydrolase activity
      • exact: pullulan 6-glucanohydrolase activity
      • exact: pullulan alpha-1,6-glucanohydrolase activity
      • broad: debranching enzyme activity
      • broad: limit dextrinase (erroneous)
    • Definition
      • Catalysis of the hydrolysis of (1,6)-a-D-glucosidic linkages in pullulan [a linear polymer of a-(1,6)-linked maltotriose units] and in amylopectin and glycogen, and the a- and b-limit dextrins of amylopectin and glycogen. [source: EC:3.2.1.41]